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研晶试剂网-试剂之家
阅读：398发布时间：2012-05-22
研晶试剂网-试剂之家
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Rabbit Anti- Vimentin抗体流式说明书, FITC conjugated
Catalog Number : RSF-0272R
Quantity size : 100ug （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. ）
Background: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas （of neural, muscle and fibroblast origin） compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Rabbit Anti- Vimentin抗体流式说明书, FITC conjugated
Specificity : • anti- Vimentin is a rabbit monoclonal antibody, FITC conjugated
• specific for Vimentin of Goat, mouse and rat
• Protein G affinity chromatography purification, purity :>95%
• Isotype: IgG
• mol wt：95kDa
Application : • FCM: 1:20-100
• Immunohistochemistry : 1:20-1:200
• Immunocytochemistry: 1:20-1:200
• Excitation spectrum: 490-495nm
• Emission spectrum: 520-530nm
• Optimal working dilutions must be determined by the end user.
• protect from light.
Rabbit Anti- Vimentin抗体流式说明书, FITC conjugated
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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