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上海研生实业有限公司
上海研生实业有限公司
阅读：810发布时间：2012-07-18
Anti-C-jun/AP-1/JUN
Catalog Number : DZ22421 Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun （previously known as the Fos binding protein p39） and c Fos form a complex in the nucleus. AP 1 （activating protein 1） is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 （cip1/waf1）, p19 （ARF） and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies..
Specificity :
– Anti-C-jun/AP-1/JUN is a rabbit monoclonal antibody unconjugated
– specific for C-jun/AP-1/JUN antibody of human,rat and mouse
– epitope mapping near the amino terminus of C-jun/AP-1/JUN antigen of human,rat and mouse
– use for western blotting, elisa, immunoprecipitation and immunohistochemistry
– Protein A affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 36kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000 Anti-C-jun/AP-1/JUN
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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