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LIF与ESGRO在细胞培养中的特点
点击次数：808 发布时间：2012-2-29
LIF与ESGRO®用于干细胞培养的优势：
1.适合胚胎干细胞、神经干细胞、造血干细胞及间充质干细胞等多种干细胞培养
2.消除了饲养层细胞的干扰与污染
3.一致地抑制ES细胞的分化
4.减少了支原体污染的危险
5.方便使用的包装形式
| 产品名称 | 产地 | 货号 | 规格 |
| ESGRO® （LIF） | Millipore | ESG1106 | 1 million units/1 mL |
| ESGRO® （LIF） | Millipore | ESG1107 | 10 million units/1 mL |
| Rat ESGRO® | Millipore | ESG2206 | 1 million units/1 mL |
| Rat ESGRO® | Millipore | ESG2207 | 10 million units/1 mL |
| Leukemia Inhibitory Factor, recombinant human | Millipore | LIF1005 | 5UG |
| Leukemia Inhibitory Factor, recombinant human | Millipore | LIF1010 | 10UG |
| Leukemia Inhibitory Factor, recombinant mouse | Millipore | LIF2005 | 5UG |
| Leukemia Inhibitory Factor, recombinant mouse | Millipore | LIF2010 | 10UG |
| Leukemia Inhibitory Factor, recombinant rat | Millipore | LIF3005 | 5UG |
| Leukemia Inhibitory Factor, recombinant rat | Millipore | LIF3010 | 10UG |
Specific Activity:
ESGRO® is assessed both on ES {embryonic stem} cells （D3 & MBL-1） and on murine M1 myeloid leukemic cells（4）. A standard of 50 Units is defined as the concentration of ESGRO®, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies（4）. 10E7 units is equivalent to approximay 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media.
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Promotional Text:
High Quality. Validated. Reliable.
EmbryoMax ESGRO® （LIF）
Description:
ESGRO® （LIF）
Trade Name:
Chemicon （Millipore）
Qty/Pk:107 units
Key Applications:
Stem Cell Culture
Application Notes:
Applications for ESGRO® include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells（E3）.
At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® is sufficient for 1.0 L of tissue culture media.
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