A90551Hu01

Polyclonal Antibody to Fibroblast Growth Factor 2, Basic （FGF2）
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

4th Edition （Revised in September, 2012）
[ PRODUCT INFORMATION ]
Immunogen:FGF2
Clonality: Polyclonal
Host:Rabbit
Species Reactivity:Human
Ig type:Rabbit IgG
Purification: Antibodies are purified by target protein affinity chromatography.
Applications: WB, ICC, IHC-P, IHC-F 
Form:Liquid
Size:100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant human FGF2 （Pro143~Ser288） expressed in E.coli.
Molecular Weight: 20.2 kDa
USCN accession No.:P90551Hu01
Sequence:The target protein is fused with two N-terminal Tags, His-tag and T7-tagand its sequence is listed below.
MGSSHHHHHHSSGLVPRGSHMASMTGGMGRGSEF-PALPEDGG SGAFPPGHFK DPKRLYCKNGGFFLRIHPDG RVDGVREKSD PHIKLQLQAE ERGVVSIKGV CANRYLAMKE DGRLLASKCVTDECFFFERL ESNNYNTYRS RKYTSWYVAL KRTGQYKLGS KTGPGQKAIL FLPMSAKS

[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na₃N, 50% glycerol.
[ STORAGE ]
Store at 4^oCfor frequent use. Stored at -20^oCto -80^oCin a manual defrost freezer for one year without detectable loss ofactivity. Avoid repeated freeze-thaw cycles.

[ ANTIBODY SPEFICITY ]
Anti FGF2is a rabbit polyclonal antibody raised against human FGF2.This antibody has been selected for its ability to recognize humanFGF2in immunohistochemical stainingandwestern blotting,noncross-reactive with other members of the family.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.