Mouse Anti-MIP-1a
Catalog Number : RS-2452R
Quantity size : 0.2ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Macrophage Inflammatory Protein 1 alpha （MIP1 alpha） and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximay 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 （C-C CKR-1） with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.
Specificity : · anti- MIP-1a is a mouse monoclonal antibody unconjugated
· specific for MIP-1a of human and rat
· epitope mapping near the N-terminus of MIP-1a of human
and rat origin
·use for western blotting,elisa,immunoprecipitation and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 20kDa
Mouse Anti-MIP-1a
Application: · Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.
Mouse Anti-MIP-1a
