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2u重组人酰基*脱氢酶8|Human ACAD8

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  • 上海高创化学科技有限公司
  • 2016-06-08 08:15:18
  • 上海市
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【简单介绍】

重组人酰基*脱氢酶8
Recombinant Human Acyl-Coenzyme A Dehydrogenase 8
Human ACAD8
Catalogue Number ENZ-294

【详细说明】

Synonyms Acyl-CoA dehydrogenase family member 8 mitochondrial, ACAD-8, Isobutyryl-CoA dehydrogenase, Activator-recruited cofactor 42 kDa component, ARC42, FLJ22590.
Introduction Acyl CoA dehydrogenase is the enzymeused to catalyzethe first step of ?-oxidationin Fatty acid metabolism.
Acyl-coenzyme A (CoA) dehydrogenases (ACADs) are a family of mitochondrial enzymes that catalyze the first dehydrogenation step in the bets-oxidation of fatty acyl-CoA derivatives. Several human ACADs exist and all ACADs catalyze the same initial dehydrogenation of the substrate at the beta-carbon atom and require electron transfer flavoprotein as an alectron acceptor. The predicted 415-amino acid ACAD8 protein contains many of the residues conserved in most other ACADs, including an active site glutamic acid residue and residues important for tetramer formation.
Description Acyl-Coenzyme A Dehydrogenase 8 Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-415 and having a total molecular mass of 47.7 kda. ACAD8 contains T7 tag at the N-terminus.
ACAD-8 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered clear solution.
Formulation Acyl-Coenzyme A Dehydrogenase 8 at a concentration of 0.1mg/ml in 10mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10mM DTT.
Stability ACAD8 although stable at 4°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Applications • ELISA.
• MS.
• Inhibition Assays.
• Western Blotting.
Purity Greater than 95.0% as determined by SDS-PAGE.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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