ELISA的方法检测多肽与小鼠前列腺特异性抗原(PSA)测定试剂盒的结合情况。准备将合成多肽(其长度大约为8~10个氨基酸)包被于ELISA板,再加入小鼠前列腺特异性抗原(PSA)测定试剂盒,一抗、酶标二抗。请问在包被的过程中应采取哪种包被缓冲液?固相载体是否要特殊处理?封闭液用BSA可以吗?
1、多肽抗原的包被一般需先使其与无关蛋白质如牛血清白蛋白质(BSA)等偶联,借助于偶联物与固相载体的吸附,间接地结合到固相载体表面。
2、小鼠前列腺特异性抗原(PSA)测定试剂盒包被用抗原或抗体的浓度,包被的温度和时间,包被液的pH等应根据试验的特点和材料的性质而选定。抗体和蛋白质抗原一般采用pH9.6的碳酸盐缓冲液作为稀释液,也有用pH7.2的磷酸盐缓冲液及pH7~8的Tris-HCL缓冲液作为稀释液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置过夜,37℃中保温2小时被认为具有同等的包被效果。
3、zui常用的封闭剂是0.05%-0.5%的牛血清白蛋白,小鼠前列腺特异性抗原(PSA)测定试剂盒也有用10%的小牛血清或1%明胶作为封闭剂的。脱脂奶粉也是一种良好的封闭剂,其zui大的特点是价廉,可以高浓度使用(5%)。
显色终止后出现黑色絮状沉淀可能为包被板上的蛋白,酶标记物,检测物,封闭液中蛋白变性以及显色反应TMB过量,不溶,沉淀出来。
小鼠前列腺特异性抗原(PSA)测定试剂盒其它优势产品如下:
klA573Mu 小鼠Ⅲ型前胶原氨基端原肽(PⅢNP)ELISA试剂盒 ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP) Assay range:30 ng/L -1161 ng/L
klA604Mu 小鼠载脂蛋白A2(APOA2)ELISA试剂盒 ELISA Kit for Apolipoprotein A2 (APOA2) Assay range:30 ng/L -1162 ng/L
klA574Mu 小鼠层粘连蛋白α1(LAMα1)ELISA试剂盒 ELISA Kit for Laminin Alpha 1 (LAMa1) Assay range:30 ng/L -1163 ng/L
klA801Mu 小鼠碱性鞘磷脂磷酸二酯酶(Alk-Smase)ELISA试剂盒 ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) Assay range:30 ng/L -1164 ng/L
klA802Mu 小鼠胆囊收缩素(CCK)ELISA试剂盒 ELISA Kit for Cholecystokinin (CCK) Assay range:30 ng/L -1165 ng/L
klA804Mu 小鼠胰高血糖素样肽1(GLP1)ELISA试剂盒 ELISA Kit for Glucagon Like Peptide 1 (GLP1) Assay range:30 ng/L -1166 ng/L
klA799Mu 小鼠骨成型蛋白7(BMP7)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 7 (BMP7) Assay range:30 ng/L -1167 ng/L
klA839Mu 小鼠促甲状腺素释放激素(TRH)ELISA试剂盒 ELISA Kit for Thyrotropin Releasing Hormone (TRH) Assay range:30 ng/L -1168 ng/L
klA843Mu 小鼠*释放激素(GnRH)ELISA试剂盒 ELISA Kit for Gonadotropin Releasing Hormone (GnRH) Assay range:30 ng/L -1169 ng/L
klA993Mu 小鼠促微管蛋白聚合蛋白(TPPP)ELISA试剂盒 ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP) Assay range:30 ng/L -1170 ng/L
klA855Mu 小鼠核因子κB受体激活因子配基(RANκL)ELISA试剂盒 ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Assay range:30 ng/L -1171 ng/L
klA372Mu 小鼠凝溶胶蛋白(GS)ELISA试剂盒 ELISA Kit for Gelsolin (GS) Assay range:30 ng/L -1172 ng/L
klA860Mu 小鼠上皮中性粒细胞激活肽78(ENA78)ELISA试剂盒 ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) Assay range:30 ng/L -1173 ng/L
klA371Mu 小鼠10kDa干扰素γ诱导蛋白(IP10)ELISA试剂盒 ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Assay range:30 ng/L -1174 ng/L
klA373Mu 小鼠结蛋白(Des)ELISA试剂盒 ELISA Kit for Desmin (Des) Assay range:30 ng/L -1175 ng/L
klB347Mu 小鼠垂体腺苷酸环化酶激活肽(ADCYAP1)ELISA试剂盒 ELISA Kit for Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) Assay range:30 ng/L -1176 ng/L
klB867Mu 小鼠表皮生长因子受体2(EGFR2)ELISA试剂盒 ELISA Kit for Epidermal Growth Factor Receptor 2 (EGFR2) Assay range:30 ng/L -1177 ng/L
klA684Mu 小鼠粘蛋白5B(MUC5B)ELISA试剂盒 ELISA Kit for Mucin 5 Subtype B (MUC5B) Assay range:30 ng/L -1178 ng/L
klB980Mu 小鼠白介素33(IL33)ELISA试剂盒 ELISA Kit for Interleukin 33 (IL33) Assay range:30 ng/L -1179 ng/L
klB154Mu 小鼠纤维蛋白原α(FGα)ELISA试剂盒 ELISA Kit for Fibrinogen Alpha (FGa) Assay range:30 ng/L -1180 ng/L
klB897Mu 小鼠胰岛素降解酶(IDE)ELISA试剂盒 ELISA Kit for Insulin Degrading Enzyme (IDE) Assay range:30 ng/L -1181 ng/L
klA753Mu 小鼠Toll样受体4(TLR4)ELISA试剂盒 ELISA Kit for Toll Like Receptor 4 (TLR4) Assay range:30 ng/L -1182 ng/L
klA663Mu 小鼠Toll样受体2(TLR2)ELISA试剂盒 ELISA Kit for Toll Like Receptor 2 (TLR2) Assay range:30 ng/L -1183 ng/L
klA295Mu 小鼠*过氧化酶1(GPX1)ELISA试剂盒 ELISA Kit for Glutathione Peroxidase 1 (GPX1) Assay range:30 ng/L -1184 ng/L
klB091Mu 小鼠肝肾骨碱性磷酸酶(ALPL)ELISA试剂盒 ELISA Kit for Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) Assay range:30 ng/L -1185 ng/L
klA479Mu 小鼠肌酸激酶MB同工酶(CKMB)ELISA试剂盒 ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB) Assay range:30 ng/L -1186 ng/L
klB697Mu 小鼠α1-抗*(α1AT)ELISA试剂盒 ELISA Kit for Alpha-1-Antitrypsin (a1AT) Assay range:30 ng/L -1187 ng/L
klB866Mu 小鼠神经调节素1(NRG1)ELISA试剂盒 ELISA Kit for Neuregulin 1 (NRG1) Assay range:30 ng/L -1188 ng/L
klB949Mu 小鼠转化生长因子β3(TGFβ3)ELISA试剂盒 ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) Assay range:30 ng/L -1189 ng/L