ELISA的方法检测多肽与小鼠水通道蛋白3(AQP-3)测定试剂盒的结合情况。准备将合成多肽(其长度大约为8~10个氨基酸)包被于ELISA板,再加入小鼠水通道蛋白3(AQP-3)测定试剂盒,一抗、酶标二抗。请问在包被的过程中应采取哪种包被缓冲液?固相载体是否要特殊处理?封闭液用BSA可以吗?
1、多肽抗原的包被一般需先使其与无关蛋白质如牛血清白蛋白质(BSA)等偶联,借助于偶联物与固相载体的吸附,间接地结合到固相载体表面。
2、小鼠水通道蛋白3(AQP-3)测定试剂盒包被用抗原或抗体的浓度,包被的温度和时间,包被液的pH等应根据试验的特点和材料的性质而选定。抗体和蛋白质抗原一般采用pH9.6的碳酸盐缓冲液作为稀释液,也有用pH7.2的磷酸盐缓冲液及pH7~8的Tris-HCL缓冲液作为稀释液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置过夜,37℃中保温2小时被认为具有同等的包被效果。
3、zui常用的封闭剂是0.05%-0.5%的牛血清白蛋白,小鼠水通道蛋白3(AQP-3)测定试剂盒也有用10%的小牛血清或1%明胶作为封闭剂的。脱脂奶粉也是一种良好的封闭剂,其zui大的特点是价廉,可以高浓度使用(5%)。
显色终止后出现黑色絮状沉淀可能为包被板上的蛋白,酶标记物,检测物,封闭液中蛋白变性以及显色反应TMB过量,不溶,沉淀出来。
小鼠水通道蛋白3(AQP-3)测定试剂盒其它优势产品如下:
klB650Mu 小鼠主要碱性蛋白(MBP)ELISA试剂盒 ELISA Kit for Major Basic Protein (MBP) Assay range:30 ng/L -1442 ng/L
klL300Mu 小鼠CD5抗原样蛋白(CD5L)ELISA试剂盒 ELISA Kit for CD5 Antigen Like Protein (CD5L) Assay range:30 ng/L -1443 ng/L
klC106Mu 小鼠骨成型蛋白10(BMP10)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 10 (BMP10) Assay range:30 ng/L -1444 ng/L
klC937Mu 小鼠鞘氨醇1磷酸酯受体1(S1PR1)ELISA试剂盒 ELISA Kit for Sphingosine 1 Phosphate Receptor 1 (S1PR1) Assay range:30 ng/L -1445 ng/L
klB792Mu 小鼠S100钙结合蛋白A8(S100A8)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Assay range:30 ng/L -1446 ng/L
klB793Mu 小鼠S100钙结合蛋白A9(S100A9)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) Assay range:30 ng/L -1447 ng/L
klJ822Mu 小鼠B-细胞κ-轻肽基因增强子抑制因子激酶β(IκBKβ)ELISA试剂盒 ELISA Kit for Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) Assay range:30 ng/L -1448 ng/L
klA549Mu 小鼠角蛋白9(KRT9)ELISA试剂盒 ELISA Kit for Keratin 9 (KRT9) Assay range:30 ng/L -1449 ng/L
klB817Mu 小鼠多功能蛋白聚糖(VS)ELISA试剂盒 ELISA Kit for Versican (VS) Assay range:30 ng/L -1450 ng/L
klC228Mu 小鼠密封蛋白(OCLN)ELISA试剂盒 ELISA Kit for Occludin (OCLN) Assay range:30 ng/L -1451 ng/L
klF292Mu 小鼠封闭蛋白2(CLDN2)ELISA试剂盒 ELISA Kit for Claudin 2 (CLDN2) Assay range:30 ng/L -1452 ng/L
klD089Mu 小鼠溶酶体酸性磷酸酶2(ACP2)ELISA试剂盒 ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2) Assay range:30 ng/L -1453 ng/L
klB280Mu 小鼠组织蛋白酶D(CTSD)ELISA试剂盒 ELISA Kit for Cathepsin D (CTSD) Assay range:30 ng/L -1454 ng/L
klB120Mu 小鼠*酶(Arg)ELISA试剂盒 ELISA Kit for Arginase (Arg) Assay range:30 ng/L -1455 ng/L
klB498Mu 小鼠蛋白二硫化物异构酶A2(PDIA2)ELISA试剂盒 ELISA Kit for Protein Disulfide Isomerase A2 (PDIA2) Assay range:30 ng/L -1456 ng/L
klM546Mu 小鼠Lin-28同源物A(LIN28A)ELISA试剂盒 ELISA Kit for Lin-28 Homolog A (LIN28A) Assay range:30 ng/L -1457 ng/L
klB884Mu 小鼠受磷蛋白(PLN)ELISA试剂盒 ELISA Kit for Phospholamban (PLN) Assay range:30 ng/L -1458 ng/L
klE676Mu 小鼠再生胰岛衍生蛋白3γ(REG3γ)ELISA试剂盒 ELISA Kit for Regenerating Islet Derived Protein 3 Gamma (REG3g) Assay range:30 ng/L -1459 ng/L
klC773Mu 小鼠兰尼定受体1(RYR1)ELISA试剂盒 ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1) Assay range:30 ng/L -1460 ng/L
klF663Mu 小鼠核因子I/B(NFIB)ELISA试剂盒 ELISA Kit for Nuclear Factor I/B (NFIB) Assay range:30 ng/L -1461 ng/L
klB564Mu 小鼠干扰素调节因子1(IRF1)ELISA试剂盒 ELISA Kit for Interferon Regulatory Factor 1 (IRF1) Assay range:30 ng/L -1462 ng/L
klH522Mu 小鼠肾连蛋白(NPNT)ELISA试剂盒 ELISA Kit for Nephronectin (NPNT) Assay range:30 ng/L -1463 ng/L
klB483Mu 小鼠胰弹性蛋白酶(ELA1)ELISA试剂盒 ELISA Kit for Elastase 1, Pancreatic (ELA1) Assay range:30 ng/L -1464 ng/L
klC010Mu 小鼠蛋白Z依赖性蛋白酶抑制因子(ZPI)ELISA试剂盒 ELISA Kit for Protein Z Dependent Protease Inhibitor (ZPI) Assay range:30 ng/L -1465 ng/L
klD696Mu 小鼠安定结合抑制因子(DBI)ELISA试剂盒 ELISA Kit for Diazepam Binding Inhibitor (DBI) Assay range:30 ng/L -1466 ng/L
klG515Mu 小鼠肥大细胞类*1(CMA1)ELISA试剂盒 ELISA Kit for Chymase 1, Mast Cell (CMA1) Assay range:30 ng/L -1467 ng/L
klB034Mu 小鼠粘蛋白4(MUC4)ELISA试剂盒 ELISA Kit for Mucin 4 (MUC4) Assay range:30 ng/L -1468 ng/L
klB226Mu 小鼠*结合球蛋白(CBG)ELISA试剂盒 ELISA Kit for Corticosteroid Binding Globulin (CBG) Assay range:30 ng/L -1469 ng/L
klB705Mu 小鼠防御素α1(DEFα1)ELISA试剂盒 ELISA Kit for Defensin Alpha 1, Neutrophil (DEFa1) Assay range:30 ng/L -1470 ng/L
klB345Mu 小鼠四旋蛋白30(TSPAN30)ELISA试剂盒 ELISA Kit for Tetraspanin 30 (TSPAN30) Assay range:30 ng/L -1471 ng/L
klA363Mu 小鼠血小板内皮细胞粘附分子1(PECAM1)ELISA试剂盒 ELISA Kit for Plaet/Endothelial Cell Adhesion Molecule (PECAM1) Assay range:30 ng/L -1472 ng/L