细胞质膜微囊蛋白-1抗体详细资料

细胞质膜微囊蛋白-1抗体详细资料

英文名称  Anti-Caveolin-1

中文名称  细胞质膜微囊蛋白-1抗体

别    名  opamine D3 receptor; Caveolin1; Caveolin 1; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1; Cav-1; ETM1; CAV 1; CAV1.

产品价格  0.1ml/100μg/ 0.2ml/200μg/

浓    度  1mg/1ml

抗体来源  Rabbit

克隆类型  polyclonal

交叉反应  hu, rat, mo

产品类型  一抗   

研究领域  信号转导 细胞凋亡 转录调节因子

蛋白分子量  predicted molecular weight: 20kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Caveolin-1 C-terminus

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

相关产品  免疫组化常用试剂    免疫印迹常用试剂    各种标记二抗 

细胞质膜微囊蛋白-1抗体详细资料参考文献  Pubmed 

产品介绍 The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq].

Function : May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.

Subunit : Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK.

Subcellular Location : Golgi apparatus membrane;Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

Tissue Specificity : Expressed in muscle and lung, less so in liver, brain and kidney.

Post-translational modifications : The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

DISEASE : Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.

Similarity : Belongs to the caveolin family.

Database links : UniProtKB/Swiss-Prot: Q03135.4

信号传导（Signaling Intermediates）

细胞质膜微囊蛋白-1抗体详细资料Caveolin-1（小凹蛋白）是细胞生长相关信号途径及肿瘤发生发展过程中重要的抑制因子，Caveolae是细胞膜内的特殊膜结构，参与包括细胞的分子运输、细胞粘附和信号转导在内的多种细胞活动。Caveolin-1是Caveolae中重要的结构蛋白，抑制细胞生长，与多种人类肿瘤发生发展相关的信号分子相互作用。Caveolin在信号转导的整合中起支架蛋白的作用。Caveolin构成了一个蛋白家族，他们是细胞质膜中发夹样结构域的主要结构成分。Caveolin在信号转导的整合中起支架蛋白的作用。至今已经鉴定了3种Caveolin （Caveolin-1、2 和3），它们具有不同的组织分布。Caveolin-1涉及上皮细胞增生的发病机制。该抗体可识别α和β型Caveolin-1。

产品图片  

Overlay histogram showing Mouse kidney cells stained with bs-1453R-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min) and then permeabilized with 1x PBS /0.2%TritonX-100 for 5 min.The cells were then incubated with the antibody (bs-1453R-PE, 2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of events were used for analysis