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技术文献:牛IgG;IgM;等ELISA试剂盒技术文献
2022-9-27 阅读(724)
【文献标题】Transcriptome analysis of the effect of pyrroloquinoline quinone disodium (PQQ·Na2) on reproductive performance in sows during gestation and lactation
【作者】Boru Zhang, Chenxi Wang, Wei Yang,et.al
【作者单位】东北农业大学（ Northeast Agricultural University）
【文献中引用产品】
牛免疫球蛋白G(IgG )ELISA试剂盒
牛免疫球蛋白A(IgA)ELISA试剂盒
牛免疫球蛋白M（IgM）ELISA试剂盒
【关键词】Oxidative stress, Pyrroloquinoline quinone, Pyrroloquinoline quinone disodium, Reproductive performance,RNA-seq, Sow
【影响因子(IF)】5.8
【出版期刊】《Journal of Animal Science and Biotechnology》
【产品原文引用】
Evaluation of antioxidant enzyme activity and the concentration of nitric oxide
Superoxide dismutase (SOD), glutathione peroxidase(GSH-Px) and catalase (CAT) enzyme activities in the milk, plasma and placenta were determined using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) in accordance with our previous study. The results of the measurements were expressed as U/mL in plasma and milk and as U/mg protein in placenta. Lipid peroxidation in the plasma,milk and placenta was determined by measuring the amounts of malondialdehyde (MDA) through the thiobarbituric acid method using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing,China). The results of the measurements were expressed as nmol/mL in plasma and as nmol/mg protein in placenta. Nitric oxide (NO) and inducible NOS (iNOS) in the plasma and placenta of sows were determined using assay kits obtained from Jiancheng Biochemistry (Nanjing, China). The colostrum was analyzed for lactose,protein, fat, and total solids with a fully automatic milk analyzer (Milko ScanTM FT+ Analyzer, Foss). The milk samples were analyzed for immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) using immunoglobulin-specific kits (Jinma Biological Engineering Co., Ltd., Shanghai, China).
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