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Description
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Application Recommended for use as a selection agent at a range of 1-10 μg/mL.
Biochem/physiol Actions Nucleoside antibiotic. Protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3′-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells. Acts very quickly and can kill 99% of cells within 2 days, the resistance gene (puromycin acetyltransferase) gives very effective protection.
Properties
assay ≥98% (TLC)
form powder
solubility H2O: 50 mg/mL (Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.)
storage temp. −20°C
Safety
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes Xn
Risk Statements 22
WGK Germany 3
RTECS AU7355000
References
reference de la Luna, S., Ortin, J., pac gene as efficient dominant marker and reporter gene in mammalian cells. Meth. Enzymol. 216, 376, (1992)
Nathans, D., Gottlieb, D., Shaw, P.D. Antibiotics New York, NY , (1967) 1, 259
Beilstein Beil. 26,IV,3704
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