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Description
Application Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Properties
composition Dye content, 90%
mp 290 °C (dec.)(lit.)
UV absorption λmax 427 nm
λmax 622 nm (2nd)
Safety
Personal Protective Equipment Eyeshields, Gloves
Hazard Codes Xn
Risk Statements 68
Safety Statements 36/37
WGK Germany 2
RTECS BQ4425000