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5ug重组小鼠β防御素2,Mouse BD-2
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- 上海高创化学科技有限公司
- 2015-12-30 17:34:47
- 上海市
- 以色列
- 412
【简单介绍】
【详细说明】
| Catalogue Number | CYT-035 | |
| Synonyms | Beta-defensin 2, BD-2, mBD-2, Defensin beta 2, Defb2, MGC129140, MGC129141. | |
| Introduction | The Defensin family are highly similar in their protein sequence and are microbicidal & cytotoxic peptides made by neutrophils. Beta Defensin-1 is an antimicrobial peptide having the resistance of epithelial surfaces to microbial colonization. Beta Defensin-1 has close proximity to Defensin Alpha-1 and has been implicated in the pathogenesis of cystic fibrosis. Skin of patients having atopic dermatitis patients and mycosis fungoides （non-lesional and lesional） show lower human Beta Defensin-1 mRNA expression and higher human Beta Defensin-2 and human Beta Defensin-3 mRNA expression. BBeta Defensin is highly expressed by epithelial cells. Beta-defensin 1 may play a role in the pathogenesis of severe sepsis. Variation in human Beta Defensin-1 contributes to asthma diagnosis, with apparent gender-specific effects. Human Beta Defensin-3 is a dimer, while Human BD-1 and Human BD-2 are monomeric. The expression of Human BD1 is correlated with induction profiles in gingival keratinocytes. The level of expression of human DEFB1 mRNA is lower than that of human BD3 and human BD-2 in reconstructed epidermis. Human BD1 is down-regulated in human prostatic and renal carcinomas. | |
| Description | Beta Defensin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.5kDa. The BD-2 is purified by proprietary chromatographic techniques. | |
| Source | Escherichia Coli. | |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation | The Mouse BD-2 was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4. | |
| Solubility | It is recommended to reconstitute the lyophilized Beta Defensin-2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability | Lyophilized Beta Defensin-2 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-2 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. | |
| Purity | Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | |
| Biological Activity | Determined by the ability to chemoattract immature human dendritic cells at a concentration of 10-100ng/ml. | |
| Amino acid sequence | AVGSLKSIGY EAELDHCHTN GGYCVRAICP PSARRPGSCF PEKNPCCKYM K. | |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |
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