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2ug重组人脑源性神经营养因子,Human BDNF
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- 上海高创化学科技有限公司
- 2016-01-10 06:08:07
- 上海市
- 以色列
- 649
【简单介绍】
【详细说明】
| Catalogue Number | CYT-207 | ||
| Synonyms | Brain-Derived Neurotrophic Factor, BDNF, MGC34632. | ||
| Introduction | BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation （ltp）, long-term depression （ltd）, certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. | ||
| Description | Brain-Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton. BDNF Human Recombinant is purified by proprietary chromatographic techniques. | ||
| Source | Escherichia Coli. | ||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | ||
| Formulation | The protein was lyophilized WITHOUT ANY ADDITIVES. | ||
| Solubility | It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | ||
| Purity | BDNF is greater than 97.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | ||
| Amino acid sequence | The sequence of the first five N-terminal amino acids of BDNF was determined and found to be Met-His-Ser-Asp-Pro. | ||
| Biological Activity | The ED50, calculated by the dose-dependent induction of ACHE （acetylcholine esterase） in rat basal forebrain primary septal culture is 50 ng/ml corresponding to a Specific Activity of 20,000IU/mg. | ||
| Protein content | BDNF quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard. | ||
| References | Title:Generation of Dopamine Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant†‡ Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press. Link:http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full | ||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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