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5ug重组人B细胞活化因子,Human BAFF
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- 上海高创化学科技有限公司
- 2015-12-30 04:46:08
- 上海市
- 以色列
- 839
【简单介绍】
【详细说明】
| Catalogue Number | CYT-307 | ||||
| Synonyms | BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor. | ||||
| Introduction | BAFF binds to tnfrsf13b/taci and tnfrsf17/bcma. Tnfsf13/april binds to the same 2 receptors, together, they form a 2 ligands -2 receptors pathway involved in the stimulation of b- and t-cell function and the regulation of humoral immunity. A third b-cell specific baff-receptor （baffr/br3） promotes the survival of mature b-cells and the b-cell response. B Lymphocyte Stimulator functions as a potent B-cell growth factor in costimulation assays. Administration of BAFF Human recombinant to mice disrupts splenic B-cell and T-cell zones and results in elevated levels of serum immunoglobulin. | ||||
| Description | BAFF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17007 Dalton. The BAFF is purified by proprietary chromatographic techniques. | ||||
| Source | Escherichia Coli. | ||||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | ||||
| Formulation | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.0. | ||||
| Solubility | It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||
| Stability | Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BAFF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. | ||||
| Purity | Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | ||||
| Amino acid sequence | MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL. | ||||
| Biological Activity | The activity is determined by a mouse splenocyte survival assay. The ED50 for this effect is 0.5-2.0µg/ml. | ||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||
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