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2ug重组大鼠血管内皮生长因子相关蛋白,Rat VEGF-C
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- 上海高创化学科技有限公司
- 2016-01-08 02:07:07
- 上海市
- 以色列
- 492
【简单介绍】
重组大鼠血管内皮生长因子相关蛋白
Rat VEGF-C
产品编号：cyt-262
【详细说明】
| Catalogue Number | CYT-262 | ||||||||||||||||||||
| Synonyms | VEGF-C, Vascular endothelial growth factor C, VRP, Flt4 ligand, Flt4-L. | ||||||||||||||||||||
| Introduction | VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein （VRP）, is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGFC cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT -4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. | ||||||||||||||||||||
| Description | Vascular Endothelial Growth Factor C Rat Recombinant contains 129 amino acids residues and was fused to a His- tag （6x His） at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions. | ||||||||||||||||||||
| Source | Sf9, Insect Cells. | ||||||||||||||||||||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | ||||||||||||||||||||
| Formulation | Each mg of VEGF-C Rat contains 50mg BSA and PBS as buffer. | ||||||||||||||||||||
| Solubility | It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||||||||||||||||||
| Stability | Lyophilized Vascular Endothelial Growth Factor-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-C should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. | ||||||||||||||||||||
| Purity | Greater than 90.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | ||||||||||||||||||||
| Biological Activity | Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells. The ED50 for this effect is typically 200-300ng/ml corresponding to a Specific Activity of 3,334-5,000IU/mg. | ||||||||||||||||||||
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||||||||||||||||||
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