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2ug重组人血管内皮生长因子B,Human VEGFB
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- 上海高创化学科技有限公司
- 2016-01-08 18:56:04
- 上海市
- 以色列
- 465
【简单介绍】
【详细说明】
| Catalogue Number | CYT-628 | ||||||||||||||||||||
| Synonyms | Vascular endothelial growth factor B, VEGF-B, VRF, VEGFL, VEGF-related factor, VEGFB. | ||||||||||||||||||||
| Introduction | VEGFB is a growth factor for endothelial cells. VEGFB 167 a.a. binds heparin and neuropilin-1 while the binding to neuropilin-1 of VEGFB 186 a.a. is regulated by proteolysis. Overexpression of VEGFB in mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. Increased VEGFB expression is associated with hepatocellular carcinoma. VEGF-B has restricted angiogenic activity in the ischemic heart. VEGFB binds to VEGFR1, Flt1, but not to VEGFR2 and VEGFR3. VEGFB is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis. | ||||||||||||||||||||
| Description | VEGFB Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 207 amino acids （22-207） and having a molecular mass of 21.6 kDa. The VEGFB is fused to a 20 amino acid His Tag at N-terminus & purified by proprietary chromatographic techniques. | ||||||||||||||||||||
| Source | Escherichia Coli. | ||||||||||||||||||||
| Physical Appearance | Sterile Filtered colorless liquid formulation. | ||||||||||||||||||||
| Formulation | The VEGFB protein solution contains 20mM Tris-HCl buffer pH 7.5 & 10% glycerol. | ||||||||||||||||||||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles. | ||||||||||||||||||||
| Purity | Greater than 90.0% as determined by SDS-PAGE. | ||||||||||||||||||||
| Biological activity | Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml corresponding to a specific activity of less than 1,000,000units/mg. | ||||||||||||||||||||
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA. | ||||||||||||||||||||
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||||||||||||||||||
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