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2ug重组人血管内皮生长因子,Human VEGF
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- 上海高创化学科技有限公司
- 2015-12-30 05:15:17
- 上海市
- 以色列
- 469
【简单介绍】
【详细说明】
| Catalogue Number | CYT-241 | ||||||||||||||||||||
| Synonyms | Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609. | ||||||||||||||||||||
| Introduction | Vascular endothelial growth factor （VEGF） is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types （e.g. stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ）. VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of the VEGF protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in the VEGF gene have been associated with proliferative and nonproliferative diabetic retinopathy. | ||||||||||||||||||||
| Description | Vascular Endothelial Growth Factor Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 38231 Dalton. The VEGF is purified by proprietary chromatographic techniques. | ||||||||||||||||||||
| Source | Escherichia Coli. | ||||||||||||||||||||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | ||||||||||||||||||||
| Formulation | The VEGF protein was lyophilized from a concentrated （1mg/ml） solution with no additives. | ||||||||||||||||||||
| Solubility | It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||||||||||||||||||
| Stability | Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | ||||||||||||||||||||
| Purity | Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | ||||||||||||||||||||
| Amino acid sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR | ||||||||||||||||||||
| Biological Activity | VEGF activity was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells （HUVEC） using a concentration range of 1-8ng/ml, corresponding to a Specific Activity of 125,000-1,000,000IU/mg. | ||||||||||||||||||||
| Protein content | VEGF protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard. | ||||||||||||||||||||
| References | Title:Expression of VEGF and Its Receptors in the Bovine Endometrium Throughout the Estrous Cycle: Effects of VEGF on Prostaglandin Production in Endometrial Cells. | ||||||||||||||||||||
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||||||||||||||||||
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