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2ug重组人酸性成纤维细胞生长因子,Human FGF-1 Sf9
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- 上海高创化学科技有限公司
- 2015-12-30 18:33:18
- 上海市
- 以色列
- 446
【简单介绍】
【详细说明】
| Catalogue Number | CYT-364 | |||||
| Synonyms | HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a. | |||||
| Introduction | Acidic fibroblast growth factor is a member of the fibroblast growth factor （FGF） family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. | |||||
| Description | Fibroblast Growth Factor-1 Human Recombinant （FGF-1） produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton. The FGF-a is purified by proprietary chromatographic techniques. | |||||
| Source | Baculovirus. | |||||
| Physical Appearance | Sterile Filtered liquid formulation. | |||||
| Formulation | The sterile protein solution （1.8mg/ml） contains 20mM Tris HCl pH=7.9, 100mM KCl, 0.2mM DTT and 20% glycerol. | |||||
| Stability | Fibroblast Growth Factor-acidic although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | |||||
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | |||||
| Amino acid sequence | The sequence of the first five N-terminal amino acids as determined and was found to be Met-Phe-Asn-Leu-Pro. | |||||
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors （measured by 3H-thymidine uptake） is <10 ng/ml, corresponding to a specific activity of 100,000IU/mg. | |||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||
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