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2ug*,Human FGF-2 Sf9
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- 上海高创化学科技有限公司
- 2015-12-23 00:42:19
- 上海市
- 以色列
- 404
【简单介绍】
【详细说明】
| Catalogue Number | CYT-365 | |||||||||
| Synonyms | Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b. | |||||||||
| Introduction | Basic fibroblast growth factor is a member of the fibroblast growth factor （FGF） family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. | |||||||||
| Description | Fibroblast Growth Factor-2 Human Recombinant （FGF-2） produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton. The FGF-basic is purified by proprietary chromatographic techniques. | |||||||||
| Source | Baculovirus. | |||||||||
| Physical Appearance | Sterile Filtered liquid formulation. | |||||||||
| Formulation | The sterile protein solution （0.5mg/ml） contains 20mM Tris pH=7.9, 100mM KCl, 1mM DTT and 20% glycerol. | |||||||||
| Stability | Fibroblast Growth Factor-basic although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | |||||||||
| Purity | Greater than 98.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | |||||||||
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile. | |||||||||
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors （measured by 3H-thymidine uptake） is <0.5 ng/ml, corresponding to a specific activity of 2MU/mg. | |||||||||
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard. | |||||||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||||||
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