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2ug重组人血小板源性生长因子-AA，Human PDGF-AA
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- 上海高创化学科技有限公司
- 2015-12-30 23:21:35
- 上海市
- 以色列
- 370
【简单介绍】
【详细说明】
| Catalogue Number | CYT-341 | |||||||||
| Synonyms | Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1. | |||||||||
| Introduction | PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in plaet alpha-granules and released upon plaet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. | |||||||||
| Description | Plaet-Derived Growth Factor AA Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 125 amino acids and having a total molecular mass of 28511 Dalton. PDGF-AA is purified by proprietary chromatographic techniques. | |||||||||
| Source | Escherichia Coli. | |||||||||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | |||||||||
| Formulation | Lyophilized 10mM Acetic Acid. | |||||||||
| Solubility | It is recommended to reconstitute the lyophilized Plaet-derived Growth Factor-AA in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |||||||||
| Stability | Lyophilized Plaet-Derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | |||||||||
| Purity | Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | |||||||||
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ile-Glu-Glu-Ala. | |||||||||
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells （measured by 3H-thymidine uptake） is < 1ng/ml, corresponding to a Specific Activity of 1,000,000units/mg. | |||||||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||||||
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