人乙酰*酯酶（AChE）酶联免疫（ELISA）试剂盒实验原理

【资料简介】

人乙酰*酯酶 （AChE）酶联免疫分析（ELISA）试剂盒仅供研究使用，用于测定人血清，血浆，组织及相关液体样本中乙酰*酯酶 （AChE）的含量。
实验原理：应用双抗体夹心法测定标本中人乙酰*酯酶 （AChE）水平。用纯化的人乙酰*酯酶 （AChE）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入乙酰*酯酶 （ACh），再与HRP标记的乙酰*酯酶 （AChE）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的乙酰*酯酶 （AChE）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中人乙酰*酯酶 （AChE）浓度。
实验注意事项：1．试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。
2．浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。
3．各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。
4．请每次测定的同时做标准曲线，做复孔。如标本中待测物质含量过高（样本OD值大于标准品孔*孔的OD值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请zui后乘以总稀释倍数（×n×5）。
5．封板膜只限一次性使用，以避免交叉污染。
6．底物请避光保存。
7．严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.
8．所有样品，洗涤液和各种废弃物都应按传染物处理。
9．本试剂不同批号组分不得混用。
10. 如与英文说明书有异，以英文说明书为准。
试剂盒保存：2-8℃。
有效期：6个月
Human Acetylcholinesterase
FOR RESEARCH USE ONLY
Drug Names
Generic Name：Human Acetylcholinesterase （AchE） ELISA Kit.
Purpose
This kit allows for the determination of AchE concentrations in Human serum, blood plasma, tissue and other biological fluids.
Principle of the assay
The kit assay Human AchE level in the sample，use Purified Human AchE antibody to coat microtiter plate wells, make solid-phase antibody, then add AchE to wells, Combined AchE antibody which With HRP labeled , become antibody - antigen - enzyme- antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AchE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher （The sample OD is bigger than the first standard well ）,please dilute Sample （n-fold）, Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Storage：2-8℃.
validity：six months.
上海华壹生物科技有限公司
Shanghai HuaYi BioTechnology Co.,Ltd
：
：021-2420 9195，136 616 74336

【备注】：仅供科学研究实验使用，以上信息仅供参考，具体产品信息以随货说明书为准。