调节激活正常T-细胞表达分泌因子（RANTES）检测试剂盒

【资料简介】

调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒

适用生物 Mus musculus (Mouse，小鼠)
调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒检测范围 78.13-5000pg/mL 灵敏度 34pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒规格 96T

ELISA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Mus musculus (Mouse)
Product No.	hzEA116Mu
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Format	96-well strip plate
Assay length	4.5 hours
Detection range	78.13-5000pg/mL The standard curve concentrations used for the ELISA’s were 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.13pg/mL
Sensitivity	The minimum detectable dose of this kit is typically less than 34pg/mL.

Specificity

This assay has high sensitivity and excellent specificity for detection of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES).
No significant cross-reactivity or interference between Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and the recovery rates were calculated by comparing the measured value to the expected amount of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	98-105	101
EDTA plasma(n=5)	85-94	88
heparin plasma(n=5)	81-98	88

调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	81-101%	88-96%	80-102%	81-97%
EDTA plasma(n=5)	82-95%	83-99%	87-94%	85-93%
heparin plasma(n=5)	88-103%	83-93%	92-99%	94-105%

调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120μL	Assay Diluent A	1×12mL
Detection Reagent B	1×120μL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37^oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37^oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37^oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37^oC;
8. Add 50μL Stop Solution. Read at 450nm immediay.

调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.