牛维生素B12（VB12）ELISA Kit

【资料简介】

牛维生素B12(VB12)ELISA Kit

产品类型:
ELISA Kit

产品名称:

牛维生素B12(VB12)ELISA Kit

英文名称:

Bovine Vitamin B12,VB12 ELISA Kit

牛维生素B12(VB12)ELISA Kit规格:
96T
牛维生素B12(VB12)ELISA Kit种属:
Bovine
待测物名称:
Vitamin B12,VB12
缩写:
VB12
蛋白功能1:
Metabolism
检测范围:
0.312pg/ml-20pg/ml
灵敏度:
0.299 pg/ml
反应时间:
1-5h
所需样本体积:
50-100ul
检测波长:
450 nm

牛维生素B12(VB12)ELISA Kit    This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.